Full Description

BS ISO 21115:2019 specifies a method for the determination of fish acute toxicity using the permanent cell line from rainbow trout (Oncorhynchus mykiss) gill, RTgill-W1. Cells in confluent monolayers in 24-well tissue culture plates are exposed to water samples, such as surface waters or different kinds of effluents, or to chemicals for 24 h and, thereafter, cell viability is assessed based on fluorescent cell viability indicator dyes (see 4.1). Data are then expressed as a percentage of unexposed control and toxicity quantified based on the percentage of cell viability versus the percentage of effluent or the chemical concentration in response curves (see Clause 9).

Cross References:
ISO 15088
OECD 236


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Product Details

Published: 04/09/2019 ISBN(s): 9780580986550 Number of Pages: 46 File Size: 1 file , 2.8 MB Same As: ISO 21115:2019 Product Code(s): 30361171, 30361171, 30361171 Note: This product is unavailable in United Kingdom